The figure shows how 6 tiny enzyme spots each mesuring 100-150 microns retain their enzymatic activity after an endogenous SS bond have been used to establish a covalent bond to a quartz surface. We have used a brief exposure to intense femtosecond UV laser pulses to open the endogenous SS bond.
The intricate interplay between photons and bio-molecules are at the very heart of biology. Whereas we have a fairly comprehensive understanding of photosynthesis-albeit not complete- our understanding of how photons may induce or modulate biochemical reactions are rudimentary at the best. When learning how to use photons in biochemical reactions we can control chemical reactions with a sequence of pulses of light. We may learn to switch on and off a particular functionality of a biocatalyst, and thereby control its function in a biosensor.
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FINAL NANOBIO PROJECTS - YEAR BASED |
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Nanobio group 9th semester projects through out the years 2007
NB3 Autumn07 Semester
Nanobio Group
The effect of UV light in Heme containing proteins - can UV light impair oxygen binding of hemoglobin?
Supervisors: Teresa Neves Petersen, Ane Kold
See project description here
 Biosensor for the detection of Reactive Oxygen Species
Supervisors: Teresa Neves Petersen, Meg Duroux, Ane Kold
See project description here
 Photodynamic Therapy-confocal microscopy of living cells
Supervisors: Teresa Neves Petersen, Meg Duroux, Ane Kold
See project description here
2006
BIOSENSORS Research Team: Meg Duroux, Esben Skosen, Laurent Duroux,
Teresa Neves Petersen, Steffen B. Petersen
I believe that light assisted immobilisation will become an essential tool in future biosensor concepts. It is clean, elegant and spatially localised. This will be come of particular importance if bio-sensors with multiple targets are sought. My research group in BioNanoPhotonics has developed such a technology.
We believe that we are excellently poised to undertake challenging collaborative projects in the area of biosensor development. We are involved in several projects of relevance for biosensor development. We have successfully developed an applicable micro-method for light assisted immobilisation onto glass, gold or silicium surfaces. A range of highly relevant protein molecules have been immobilised and retained their activity. We have extensive background in doing biomolecular spectroscopy, monitoring activity and stability. We have developed our own proprietary bioinformatics tools, including ways of modelling protein electrostatics, as a function of environmental parameters such as pH and electrostatic fields.
LIGHT USED TO IMMOBILISE BIOMOLECULES.
Sensors will continue to dramatically extend and enhance our ability to grasp realities far beyond our own sensory apparatus. Most likely it will fundamentally change the way we perceive the world we live in. Biosensors will become a fundamental modality in the health care sector in the modern society. The possibilities for making functional bio-sensors detecting specific molecules are virtually endless. I believe that Nanotechnology combined with clever innovative use of biomolecules will pave the way for very exciting totally new bio-sensor types. Light and Biomolecules - UltraFastBiospectrocopy
LIGHT AND BIOMOLECULES
Research Team: Teresa Neves Petersen, Esben Skosen, Søren Klitgaard, Steffen B. Petersen
The study of light induced reaction always fascinated the curious minds: they are ultrafast, can be studied using ultrafast laser systems, can be controlled by pulsed laser light. Light induced reactions have lead to life as we know it now!! Nature used light to initiate reactions and is our task to discover its mechanisms and develop new applications. We study the light induced reactions using the latest femtosecond laser system. Detection can be done in a single photon counting mode, using streak camera as a detector or pump probe spectroscopy that allows us to follow the dynamics of reaction mechanisms with fs resolution. Our studies on light induced reactions goes hand-in-hand with bioinformatic and protein structure analyses. These studies give us the necessary insight necessary to predict and understand the molecular mechanisms behind the photo-induced reactions.
Research Team: Teresa Neves Petersen, Søren Klitgaard, Steffen B. Petersen
New Photodynamic Cancer Therapy - Light Induced Cancer Cell Death
Research Team: Teresa Neves Petersen, Søren Klitgaard, Steffen B. Petersen
  The effects of the new technology have been predictedby Prof Steffen Petersen and Teresa Neves Petersen and subsequently confirmed in two carcinogenic cell lines, in a joint collaboration between the University of Aalborg (AAU) and the University of Southern Denmark (SDU).  The newly discovered UV-light induced reaction has already proven to be of use in stopping cancer cell proliferation and induce cancer cell apoptosis.
Link to our publication in the "International Journal of Oncology" here
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