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Regarding all books, check if available at the central bookstore. Books available at Amazon UK. Contact the course responsible if needed.
All 6th semester courses notes ahead
Courses taught by the Nanobio group
Course: Biosensors
Lectors: Leonid Gureovich, Teresa Neves Petersen
Book: Brian Eggins, Chemical Sensors and Biosensors with some selected chapters from Cooper and Cass, “Biosensors. Practical Approach”
Plus notes that each lector hands in during the course
5 Lectures taught by Teresa Neves Petersen
Course timing:
10April
14 April - 1pm
17April - 9am
21 April - 1pm
28April - 1pm
Local: Room 1.212
Course structure:
Lecture 1 - Definition of Luminescence and the different forms of luminescence illustrated with ley examples. Theoretical principles of fluorescence and phosphorescence (fast review from 4th semester concepts). Chemiluminescence: principles and demosntration. Bioluminescence: molecular structural insight of reactions leading to bioluminescence. The fate of the excited molecular state. The use of fluorescence, chemiluminescence and bioluminescence in biosensor technology. Excitation and detection sources: PMT, CCD, streak camera, optical fibers. Applications: detection of blood metabolites, detection of peroxides.
Notes Lecture 1 - here
Problems - here
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Lecture 2 - Biosensor technology as a must in aging societies, desease prevention and societal costs. New concept of health monitoring and health care in the 21st century: why is it needed, how to implemente it, its benefits to the individual and to the society in genreral. Biosensor plattform technology, Biosensot definition, generics of a biosensor and applications. Principles of signal detection in a biosensor assay: QCM-D, ATR-FTIR, CMOS biosensor, TIRF based biosensors plus fast review of TIRF principle (4th semester knowledge).
Microarray: from prodution to a result. Characteristics of microarrays. Microarrays experiemtal workflow: microarray fabrication, microarray processing and microarray analyse. Microarray fabrication: description of different immobilisation techniques and visualisation in the form of films, of different liquid dispending technologies used nowadays to fabricate microarrays. Microarray processing - Initiated the discussion around this second major task of the experimental workflow.
Notes Lecture 2 - here
Problems - here
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Lecture 3 - Microarrays experiemtal workflow
Microarray processing - Elisa like sandwich assay, Displacement assay. Working principle of each assay. Learning how to quantify the analyte concetration
Microarray analyses - labelling sensors with fluorophores. Structure of fluoroplhores and its spectral characteristics. Learning how to choose the correct dye for the correct instrument used to image the microarrays. Fluorecence microscopy vs laser scanner: resolution, filters, dyes, operation principles. Laser scanner: animation showing the operating principle. Confocal vs non-confocal scanning.
Data analyses - Judging the goodness of an array prior and after analyte binding. Important statistical parameters that we need to recover from an array and how to plot such information as histograms. Multiple testing concept: challenges and benefits. Improved diagnostic quality with multiplexed sensing.
Notes Lecture 3 - here
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Lecture 4 - Plasmonic biosensors - engineering the radiative decay, Jablonski diagram in the presence of metal layour, effect of metal layour on fluorescence quantum yield, fluorescence lifetime. SPCE - surface plasmon coupled emissoin, Gold and silver islands and SPCE. Experimental layout: comparison and discussion. Directional emission vs isotropic fluorescence emission. Biological and Biomedical applications. What is a plasmon and a polariton. Plasmon ressonance frequency. Choice of fluorophore and metal in order to achieve SPCE. Absorption spectra of different plasmonic surfaces. Experimental setup needed for SPCE. Surface Plamon Ressonance . SPR
Notes Lecture 4 - here
Problems - Problems are included in the lecture notes. Additional problems derived from discussion during lecture 3 are included here
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Lecture 5
Notes Lecture 5
Problems -
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